Botox uygulaması bireyden bireye farklılık göstermektedir. Kişilerin hücre yenileme hızlarına bağlı olarak bu süreler değişiklik göstermektedir. Genel yapılan araştırma ve sonuçlara bakıldığı zaman Botox etkisi 7-8 ay kadar kalıcılığını korumaktadır.
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